Fig. 5
From: BRCA1 mutation influences progesterone response in human benign mammary organoids
Expression of ALDH1 and cell type-specific genes in BRCA1mut and non-carrier mammary organoids. a Immunofluorescent staining was done in BRCA1mut and non-carrier organoids with ALDH1 (red) and DAPI (blue) to visualize the nuclei. Scale bar, 100 μm. b Normalized expression of ALDH1 mRNA in individual BRCA1mut and non-carrier mammary organoids is shown (N = 4, p = 0.1106, unpaired t-test). c–f Normalized reads in individual BRCA1mut and non-carrier organoids treated with E2+P4 are shown in the left panels for c luminal HR-positive genes, d luminal HR-negative genes, e myoepithelial genes, and f fibroblasts genes. The effect of TPA treatment in BRCA1mut and non-carrier organoids are shown as fold changes of hormone treatment (red dotted line = 1) in the right panels for each cell-specific genes (*p < 0.05, **p < 0.01, ***p < 0.001)