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Fig. 4 | Breast Cancer Research

Fig. 4

From: BRCA1 mutation influences progesterone response in human benign mammary organoids

Fig. 4

Confirmation and validation of ECM genes. a Confirmation of ECM genes was done by real-time PCR analysis of the samples used in the RNA-seq analysis. The data are fold changes of hormone treatment (dotted red line) and represent the mean ± SEM from three technical replicates (*p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001). b Validation of ECM genes was done by real-time PCR using new patient-derived individual BRCA1mut and non-carrier organoids treated with E2+P4 and E2+P4+TPA. The data are fold changes of hormone treatment (dotted red line) and represent the mean ± SEM from three technical replicates (*p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001. c IHC staining of ECM proteins MMP10, COL1A1, COL3A1, and FN1 were performed in non-carrier and BRCA1mut organoids. Scale bar, 100 μm

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