Fig. 2
From: BRCA1 mutation influences progesterone response in human benign mammary organoids
Characterization of mammary organoids. a IHC staining was done for PR (brown), epithelial marker pan CK (brown), luminal marker CK18 (brown), fibroblast marker vimentin (red), and basal marker αSMA (red) in non-carrier and BRCA1mut breast tissue. Scale bar, 100 μm. b Left, H&E staining, Right, trichrome staining for collagen fibers (blue) is shown for BRCA1mut and non-carrier organoids. c Immunofluorescent staining was done for E-cadherin (E-cad), red, a cell-cell adhesion marker and Integrin beta 4 (ITGB4), green, a cell-ECM adhesion marker in BRCA1mut and non-carrier organoids. d Left, Immunofluorescent co-staining of BRCA1mut organoid with luminal marker CK18 (red) and basal marker CK5/6 (green). Myoepithelial/basal marker αSMA (red) and fibroblast marker vimentin (green) is shown. Right panel is immunofluorescent co-staining of non-carrier organoids with luminal marker CK18 (red) and myoepithelial/basal marker αSMA (green), and myoepithelial/basal marker αSMA (red) and fibroblast marker vimentin (green). Scale bar, 100 μm