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Fig. 8 | Breast Cancer Research

Fig. 8

From: CGRRF1, a growth suppressor, regulates EGFR ubiquitination in breast cancer

Fig. 8

CGRRF1 regulates AKT phosphorylation and the expression of nuclear EGFR. a, b AKT phosphorylation after EGF stimulation in CGRRF1-knockout MCF7 cells (a) and MDA-MB-231 cells (b) was examined by western blot analysis. c EGF-induced AKT phosphorylation in CGRRF1-overexpressing BT-549 cells was examined by western blot analysis. The quantification of AKT phosphorylation is shown in the bottom graphs. df Subcellular fractionation experiments were performed to examine the levels of nuclear EGFR in CGRRF1-overexpressing BT-549 cells (d), CGRRF1-overexpressing MDA-MB-231 cells (e), and CGRRF1-knockdown MDA-MB-231 cells (f). The experiment in e was carried out under 2% serum condition to increase the expression of mutant CGRRF1. p84 was used as a nuclear marker, and GAPDH was used as a cytosolic marker. We also harvested parallel plates for total protein lysates (right panels of df). Nonspecific bands are marked with asterisks (*)

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