Fig. 3

CGRRF1 inhibits the growth of breast cancer in vivo. a The stably transduced MDA-MB-231 cell lines as indicated were used to establish xenografts in NSG mice. Tumors were harvested on day 26. Left panel: tumor volumes; right panel: weights of xenografts. The final tumor volumes and tumor weights were calculated by averaging both sides of tumors. There was one mouse in the pLenti group which was only injected on one side. N represents the total number of tumors harvested. Error bars represent mean ± SE (*p < 0.05, N.S.: not significant). b The expression of CGRRF1 in xenografts was verified by western blot analysis. The asterisk (*) labels a band recognized by CGRRF1 antibody, which could be a degradation product or nonspecific staining. c Box plot of the levels of EGFR protein in xenografts obtained from RPPA analysis. The EGFR level was shown relative to the average of the pLenti group. *p < 0.05. d Box plot of the levels of EGFR in xenografts verified by western blot analysis. The EGFR level was normalized by loading control vinculin and then shown as relative to the average of pLenti xenografts (western blot is shown in Additional file 3: Figure S3C). *p < 0.05