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Fig. 1 | Breast Cancer Research

Fig. 1

From: CGRRF1, a growth suppressor, regulates EGFR ubiquitination in breast cancer

Fig. 1

Knockdown of CGRRF1 enhances the growth of breast cancer cell lines. a Western blot analysis of CGRRF1 in a panel of breast cancer cell lines with different estrogen receptor status. The intensities of CGRRF1 and loading control β-actin in each lane were quantified using the Odyssey CLx infrared imaging system, and the signals of CGRRF1 were normalized by the corresponding β-actin signals and shown as relative to that of MCF7 cells. b The growth rate of CGRRF1-knockdown MCF7 cell lines was determined by MTT assay. Error bars represent mean ± SD (n = 5). *p < 0.01. Knockdown of CGRRF1 was confirmed by western blot analysis (lower panel). c The growth rates of CGRRF1-knockdown SKBR3 cell lines were determined by MTT assay. Error bars represent mean ± SD (n = 3). **p < 0.001. Knockdown of CGRRF1 was confirmed by western blot analysis (right panel). d The growth rates of CGRRF1-knockdown BT-549 cell lines were determined by MTT assay. Error bars represent mean ± SD (n = 9). *p < 0.01. Knockdown of CGRRF1 was confirmed by western blot analysis (lower panel). A nonspecific band was marked with an asterisk (*). e Clonogenic cell survival assay of CGRRF1-knockdown BT-549 cell lines. Error bars represent mean ± SD (n = 3). *p < 0.01. (A complete set of colony formation is presented in Additional file 1: Figure S1A.)

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