Fig. 7

Effect of an IL6 receptor blocking antibody on tumor growth. a qPR-PCR analysis of IL6 expression levels in SUM159 xenografts. b Female nude mice were injected as described in Fig. 5 using the SUM159+C and SUM159+P cells, which produced the largest tumors. Upon detection of a palpable tumor, an intratumoral injection of an IL6 receptor blocking antibody or non-specific IgG control was injected every day till study end. c Tumor initiation over time for each cell line in the presence of the IL6 receptor blocking antibody or control. Data are from one representative experiment (mean ± SD) of two independent experiments, n = 8 mice/treatment group for a and n = 4 mice/treatment group for b, c. d Representative images of cells were grown in Matrigel +/− treatment with an IL6 receptor blocking antibody or non-specific IgG control (added fresh each day) and growth imaged over time. +C, CRYβB2 overexpression; +P1, CRYβB2P1 overexpression; P-/-, CRYβB2P1 knockout; C-/-, CRYβB2 knockout; +C+P, CRYβB2 and CRYβB2P1 dual overexpression