Fig. 4

Effect of CRYβB2 and CRYβB2P1 expression on tumorigenicity. a Adult female nude mice were injected into the right abdominal mammary gland with the indicated SUM159 models and orthotopic tumor growth measured over time. b qRT-PCR analysis of MKI67 (expression levels within the tumors). c Tumor initiation represented as percent of mice tumor free over time. 100% signifies no mice have tumors, 0% indicates all mice have tumors. Data from one representative experiment of three independent experiments, n = 5 to 8 mice per treatment group, *p < 0.005, **p < 0.05 compared to SUM159. d, e qRT-PCR expression levels of the indicated genes in primary xenograft tumor samples. f, g Detection of human cells within common metastatic sites in xenograft models. Cells were detected via qRT-PCR for the human-specific gene β-2-microglobulin. +C, CRYβB2 overexpression; +P1, CRYβB2P1 overexpression; P-/-, CRYβB2P1 knockout; C-/-, CRYβB2 knockout; +C+P1, CRYβB2 and CRYβB2P1 dual overexpression