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Fig. 3 | Breast Cancer Research

Fig. 3

From: A functional role for the cancer disparity-linked genes, CRYβB2 and CRYβB2P1, in the promotion of breast cancer

Fig. 3

Expression and proliferation of SUM159 and Hs578t models including double overexpression of CRYβB2/CRYβB2P1 and CRYβB2 knockout. ad qRT-PCR analysis of the indicated cell lines with altered CRYβB2 and CRYβB2P1. Overexpression and knockout clonal populations were generated and selected via lentiviral transduction and CRISPR/Cas9-mediated transfection, flow cytometry, and antibiotic selection. The indicated gene transcript abundance was measured via qRT-PCR. e, f Proliferation assays: cells were plated at 25,000 cells per well in triplicate and counted every 24 h for 96 h. All data are mean ± SE of a minimum of three independent experiments. *p < 0.05. +C, CRYβB2 overexpression; +P1, CRYβB2P1 overexpression; P-/-, CRYβB2P1 knockout; C-/-, CRYβB2 knockout; +C+P, CRYβB2 and CRYβB2P1 dual overexpression

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