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Fig. 6 | Breast Cancer Research

Fig. 6

From: Calcium-sensing stromal interaction molecule 2 upregulates nuclear factor of activated T cells 1 and transforming growth factor-β signaling to promote breast cancer metastasis

Fig. 6

Effects of NFAT1 on TGF-β1 expression and secretion. a Ingenuity Pathway Analysis summary. Using IPA, we identified downstream molecules of NFAT1, including TGF-β1, a key regulator of EMT. b Results of ELISA showing the secretion of TGF-β1 with knockdown (SH), overexpression (OE), or rescue of NFAT1 and STIM2. c Results of dual-reporter luciferase assay showing NFAT1-dependent activation of the TGF-β1 promoter. Reporter gene constructs were transfected into HEK 293 T cells, and luciferase activity was measured after 48 h. d Chromatin immunoprecipitation of NFAT1 on the TGF-β1 promoter. After cells were lysed, proteins were cross-linked and immunoprecipitated with an anti-NFAT1 antibody. The cross-linking was reversed, and PCR was performed. One of 3 separate experiments is shown. e Signaling pathway formed by STIM2, NFAT1, and TGF-β1 was showed. T tests or one-way ANOVA were used to compare independent groups. Data shown are means ± SD from at least 3 independent experiments. NC, control. *P < 0.05

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