Fig. 5

NFAT1 regulates EMT in breast cancer cells. a Western blots (left) and quantification (right) showing knockdown (SH) or overexpression (OE) of NFAT1 in MDA-MB-231 cells. b Western blots (left) and quantification (right) of expression of the EMT marker proteins vimentin in MDA-MB-231 cells with knockdown or overexpression of NFAT1. c Confocal images of immunofluorescence staining (× 100) of vimentin in MDA-MB-231 cells with knockdown or overexpression of NFAT1. d Images (right) and quantification (left) of Transwell assay showing migration of MDA-MB-231 cells with NFAT1 knockdown or overexpression. T tests or one-way ANOVA were used to compare independent groups. Data shown are means ± SD of at least 3 independent experiments. *P < 0.05. NC, control. e Western blots (left) and quantification (right) showing rescue of vimentin and E-cadherin expression in MDA-MB-231 and MCF7 cells with STIM2 knockdown (SH) and/or overexpression (OE) of NFAT1. f Images and quantification of transwell assays showing migration of MDA-MB-231 cells with STIM2-SH/NFAT1-OE and MDA-MB-231 cells with NFAT1-SH/STIM2-OE. g Effects of knockdown or overexpression of NFAT1 on the transcription of ZEB1, TWIST1, SNAI1, SNAI2, SMAD4, and SMAD5, in MDA-MB-231 cells. T tests or one-way ANOVA were used to compare independent groups. Data shown are means ± SD of at least 3 independent experiments. *P < 0.05. NC, control