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Fig. 3 | Breast Cancer Research

Fig. 3

From: Calcium-sensing stromal interaction molecule 2 upregulates nuclear factor of activated T cells 1 and transforming growth factor-β signaling to promote breast cancer metastasis

Fig. 3

STIM2 regulates EMT in breast cancer cells. a Western blots (left) and quantification (right) showing knockdown (SH) or overexpression (OE) of STIM1 or STIM2 in MCF7 cells transfected with lentivirus. NC, control. b Western blots (left) and quantification (right) showing expression levels of the epithelial-mesenchymal transition (EMT) marker proteins E-cadherin in MCF7 cells and vimentin in MDA-MB-231 cells after knockdown or overexpression of STIM1 or STIM2. c Representative immunofluorescence images showing E-cadherin in MCF7 and vimentin in MDA-MB-231 cells with knockdown or overexpression of STIM2. d Western blots (left) and quantification (right) showing repression (rescue) of STIM2 in MDA-MB-231 cells with STIM2 knockdown and the impact of STIM2 repression on expression of vimentin. e mRNA expression of ZEB1, TWIST1, and SNAI1 were measured by qRT-PCR in MDA-MB-231 cells with STIM2 knockdown or overexpression. f Photomicrographs (left) and quantification (right) of Transwell assays showing migration of MDA-MB-231 cells with knockdown or overexpression of STIM2. T tests or one-way ANOVA were used to compare independent groups. Data shown are means ± SD from at least 3 independent experiments. *P < 0.05

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