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Fig. 4 | Breast Cancer Research

Fig. 4

From: Investigating circulating tumor cells and distant metastases in patient-derived orthotopic xenograft models of triple-negative breast cancer

Fig. 4

Representative fluorescent images of CTCs isolated from different PDOX tumor-bearing mice. Live CTCs were isolated from individual PDOX tumor-bearing animals by label-free Vortex technology. Captured cells were then fixed, permeabilized, and stained for human cytokeratin (CK), human vimentin (VIM), and mouse CD45. Nuclei were highlighted with DAPI. CTCs were identified as DAPI-positive cells that were positive for cytokeratin and/or vimentin and negative for CD45 (to exclude mouse hematopoietic cells)

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