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Fig. 6 | Breast Cancer Research

Fig. 6

From: Neoadjuvant neratinib promotes ferroptosis and inhibits brain metastasis in a novel syngeneic model of spontaneous HER2+ve breast cancer metastasis

Fig. 6

Impact of neratinib on HER2 signalling and iron metabolism in TBCP-1 tumours. Mice with palpable tumours were treated daily with vehicle or neratinib (60 mg/kg) for 4 days and tumours resected 2 h after the last neratinib dose. a Tumour lysates were analysed for the expression of total or phosphorylated EGFR, HER2, MAPK and AKT by western blotting as indicated and the protein bands quantitated by densitometry as described in the “Methods” section. The results are expressed as the ratio of phospho/total protein. b Markers of ferroptosis/iron metabolism were analysed by western blotting as described above and band intensity normalised to GAPDH. Each point in a and b represents one mouse and data show mean ± SEM of four control or neratinib-treated tumours (n = 4). *p < 0.05

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