Fig. 3

miR-671-5P inhibits proliferation in a time- and dose-dependent manner in the 21T cell lines. The 21T cell lines were transfected with mock control, miR-671-5p mimic, and inhibitor control and miR-671-5p inhibitor. a The proliferation rate was decreased after transfection of miR-671-5p mimic in 21NT and 21MT, while there were no significant changes in 21PT and H16N2 cells lines compared to the mock control (top panel). Inversely, transfection of miR-671-5p inhibitor significantly promoted the proliferation in 21MT while slightly in 21NT, but not in H16N2 and 21PT compared to the inhibitor mock (middle panel). The T21 cell lines were co-transfected with miR-671-5p mimic and pcDNA3.1-FOXM1 or pcDNA3.1 empty vector. b Forced expression of FOXM1 rescued miR-671-5p-mediated cell proliferation in a dose- and time-dependent manner. Values represent the mean ± S.D. for three independent experiments (*p < 0.05 and **p < 0.01)