Table 1 Lists of antibodies used for flow cytometry and western blotting
From: Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer
Antigen | Company | Clone | Catalog no. | Â |
|---|---|---|---|---|
Antibodies for cell sorting | ||||
 CD44 | BD Biosciences | G44-26 (C26) | 563029 |  |
 CD142 | BD Biosciences | HTF-1 | 561713 |  |
 CD144 | BD Biosciences | 55-7H1 | 561714 |  |
 CD146 | BD Biosciences | P1H12 | 561013 |  |
 CD171 | Thermo Fisher Scientific | eBio5G3 (5G3) | 17-1719-41 |  |
 CD220 | BD Biosciences | 3B6/IR | 559955 |  |
 CD221 | BD Biosciences | 1H7 | 560934 |  |
 ERBB1 | BD Biosciences | EGFR.1 | 563344 |  |
 SSEA-1 | BD Biosciences | MC480 | 560886 |  |
 Mouse IgG1 κ | Thermo Fisher Scientific | P3.6.2.8.1 | 12-4714-42 | Isotype Control |
 Mouse IgG2a κ | BD Biosciences | G155-178 | 550882 | Isotype Control |
 Mouse IgG2b κ | BD Biosciences | 27-35 | 563025 | Isotype Control |
 Mouse IgM κ | BD Biosciences | G155-228 | 555584 | Isotype Control |
Primary antibodies for western blotting | ||||
 COX2 | Cell Signaling Technology | D5H5 | 12282 |  |
 MMP2 | Cell Signaling Technology | D8N9Y | 13132 |  |
 MMP14 | Cell Signaling Technology | D1E4 | 13130 |  |
 p-SMAD2 (S465/467)/SMAD3 (S423/425) | Cell Signaling Technology | D27F4 | 8828 |  |
 SMAD2/3 | Cell Signaling Technology | D7G7 | 8685 |  |
 p-EPHA4 (Y596) | Abcam | Polyclonal | ab193214 |  |
 EPHA4 | Abcam | Polyclonal | ab126169 |  |
 p-EPHA2 (S897) | Cell Signaling Technology | D9A1 | 6347 |  |
 EPHA2 | Cell Signaling Technology | D4A2 | 6997 |  |
 VEGFR2 | Santa Cruz Biotechnology | A-3 | sc-6251 |  |
 ACTB | Cell Signaling Technology | D6A8 | 12620 |  |
 ERBB1 | Cell Signaling Technology | D38B1 | 4267 |  |
 FGFR1 | Santa Cruz Biotechnology | M2F12 | sc-57132 |  |
 FGFR2 | Cell Signaling Technology | D4L2V | 23328 |  |
 IGF1R | Cell Signaling Technology | D23H3 | 9750 |  |
 VEGFR1 | Santa Cruz Biotechnology | D-2 | sc-271789 |  |
Secondary antibodies for western blotting | ||||
 Mouse IgG | Jackson ImmunoResearch |  | 715-036-151 |  |
 Rabbit IgG | Cell Signaling Technology |  | 7074 |  |