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Table 1 Lists of antibodies used for flow cytometry and western blotting

From: Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer

Antigen Company Clone Catalog no.  
Antibodies for cell sorting
 CD44 BD Biosciences G44-26 (C26) 563029  
 CD142 BD Biosciences HTF-1 561713  
 CD144 BD Biosciences 55-7H1 561714  
 CD146 BD Biosciences P1H12 561013  
 CD171 Thermo Fisher Scientific eBio5G3 (5G3) 17-1719-41  
 CD220 BD Biosciences 3B6/IR 559955  
 CD221 BD Biosciences 1H7 560934  
 ERBB1 BD Biosciences EGFR.1 563344  
 SSEA-1 BD Biosciences MC480 560886  
 Mouse IgG1 κ Thermo Fisher Scientific P3.6.2.8.1 12-4714-42 Isotype Control
 Mouse IgG2a κ BD Biosciences G155-178 550882 Isotype Control
 Mouse IgG2b κ BD Biosciences 27-35 563025 Isotype Control
 Mouse IgM κ BD Biosciences G155-228 555584 Isotype Control
Primary antibodies for western blotting
 COX2 Cell Signaling Technology D5H5 12282  
 MMP2 Cell Signaling Technology D8N9Y 13132  
 MMP14 Cell Signaling Technology D1E4 13130  
 p-SMAD2 (S465/467)/SMAD3 (S423/425) Cell Signaling Technology D27F4 8828  
 SMAD2/3 Cell Signaling Technology D7G7 8685  
 p-EPHA4 (Y596) Abcam Polyclonal ab193214  
 EPHA4 Abcam Polyclonal ab126169  
 p-EPHA2 (S897) Cell Signaling Technology D9A1 6347  
 EPHA2 Cell Signaling Technology D4A2 6997  
 VEGFR2 Santa Cruz Biotechnology A-3 sc-6251  
 ACTB Cell Signaling Technology D6A8 12620  
 ERBB1 Cell Signaling Technology D38B1 4267  
 FGFR1 Santa Cruz Biotechnology M2F12 sc-57132  
 FGFR2 Cell Signaling Technology D4L2V 23328  
 IGF1R Cell Signaling Technology D23H3 9750  
 VEGFR1 Santa Cruz Biotechnology D-2 sc-271789  
Secondary antibodies for western blotting
 Mouse IgG Jackson ImmunoResearch   715-036-151  
 Rabbit IgG Cell Signaling Technology   7074