Skip to main content

Advertisement

Fig. 3 | Breast Cancer Research

Fig. 3

From: Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer

Fig. 3

Tzm loading partially induced vascular features in HER2+ breast cancer cells. a Tzm sensitivity assay. SKBR3 and BT474 cells positive and negative for each cell surface antigen were cultured in the presence or absence of Tzm for 6 days, and cell counts relative to those of Tzm-absent controls are shown (n = 4; mean ± SD). *P < 0.05; **P < 0.01. b Western blots of VM markers in cells loaded with IgG or Tzm for 13 days. HUVECs served as positive controls for FGFR1, VEGFR1, and VEGFR2 expression. *, IGF1R protein. c Fold changes in mRNA expression of VM markers in cells loaded with Tzm for 13 days relative to control cells as determined by qRT-PCR. mRNA expression was determined by the 2−∆∆Ct method using TaqMan probes (n = 3; mean ± SD). d Tube formation assay using SKBR3 and BT474 cells precultured with IgG or Tzm for 13 days. Representative photographs are shown. Scale bar, 0.5 mm

Back to article page