The prognostic value of JUNB-positive CTCs in metastatic breast cancer: from bioinformatics to phenotypic characterization

Table 3 JUNB expression in primary and metastatic tumors

Patient	Material	JUN B IHC EVAL - FFPE tissues			JUNB positive (+) CTCs
Patient	Material	% (0–100)	Intensity (0–3)	H-score* (0–300)	JUNB positive (+) CTCs
1	Primary tumor	0	0	0	–
2	Primary tumor	0	0	0	+
3	Primary tumor	3	2	6	+
4	Primary tumor	5	1	5	Negative for CTCs
5	Primary tumor	0	0	0	Negative for CTCs
6	Primary tumor	0	0	0	Negative for CTCs
7	Primary tumor	0	0	0	Negative for CTCs
8	Primary tumor	9	1	9	–
9	Primary tumor	0	0	0	Negative for CTCs
10	Primary tumor	0	0	0	Negative for CTCs
11	Primary tumor	0	0	0	+
11	Metastasis	40	3	120	+
12	Metastasis	10	2	20	Negative for CTCs
13	Metastasis	35	3	105	+
14	Metastasis	40 &20	2&3	140	+

*Histoscore (H-score) was calculated by a semi-quantitative assessment of both the intensity of staining (graded as: 0: no-staining; 1: weak; 2: medium; or 3: strong) and the percentage of positive cells. The percentage of cells at each staining intensity level was calculated, and an H-score was assigned summming the individual H-scores for each intensity level using the following formula: [1 × (% cells 1+) + 2 × (% cells 2+) + 3 × (% cells 3+)]. The range of possible scores was from 0 to 300

ISSN: 1465-542X