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Fig. 4 | Breast Cancer Research

Fig. 4

From: Glucocorticoid receptor modulation decreases ER-positive breast cancer cell proliferation and suppresses wild-type and mutant ER chromatin association

Fig. 4

GR activation inhibits constitutively-active Y537S ER-mediated proliferation and ER chromatin association. MCF-7 HA-tagged Y537S cells were pre-treated for 48 h with 0.5 μg/mL doxycycline to induce the expression of HA-tagged Y537S ER. a Cells were treated with GR-selective ligands for 3 and 6 days. Dex, C134, and C335 significantly inhibited mutant Y537S ER-mediated proliferation (**p < 0.01, ***p < 0.001, ****p < 0.0001, two-way ANOVA, Tukey’s post hoc test, n = 3, ±SD). B) Cells were treated with GR-selective ligands for 4 or 6 h, and CCND1, CDK2, and CDK6 gene expression was reduced following GR liganding with Dex, C134, or C335 (*p < 0.05, **p < 0.01, ****p < 0.0001, two-way ANOVA, Tukey’s post hoc test, n = 3, ±SD)

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