Fig. 6
From: Gene expression signature of atypical breast hyperplasia and regulation by SFRP1
Expression of genes in AH and regulation by SFRP1. Genes associated with AH were identified by AGNES and PAM were examined for regulation by SFRP1. a The relative expression of the genes in AH are shown. Green indicates genes with decreased expression in AH relative to the HNB tissues and red indicates those with increased expression. b The effect of SFPR1 knockdown on gene expression was analyzed in a cell line derived from normal breast epithelium and immortalized with telomerase (76NTERT cells). Relative levels of transcripts were determined by RT-qPCR in TERT-siSFRP1 cells and the TERT-pSUPER vector control cells. c Similarly, mRNA levels of the genes was compared by RT-qPCR in mouse mammary glands derived from Sfrp1−/− and control Sfrp1+/+ mice. d Breast cancer cells overexpressing SFRP1 (MCF7-SFRP1) and control cells (MCF7-pCDNA) were also compared for relative levels of gene expression by RT-qPCR. The level of SFRP1 mRNA was normalized to the amplification of ACTB mRNA, which was performed in parallel wells for each cell line or tissue. Bars represent mean ± SEM SFRP1/ACTB and are expressed as relative expression of control TERT-pSUPER (b), control mice (c), and MCF7-pCDNA (d). Genes where loss of SFRP1 results in decreased expression are shown in green, those increased are shown in red, and those which are unchanged are shown in white *p < 0.05, **p < 0.01, and ***p < 0.001 (significantly different from control using Student’s t test)