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Fig. 6 | Breast Cancer Research

Fig. 6

From: Gene expression signature of atypical breast hyperplasia and regulation by SFRP1

Fig. 6

Expression of genes in AH and regulation by SFRP1. Genes associated with AH were identified by AGNES and PAM were examined for regulation by SFRP1. a The relative expression of the genes in AH are shown. Green indicates genes with decreased expression in AH relative to the HNB tissues and red indicates those with increased expression. b The effect of SFPR1 knockdown on gene expression was analyzed in a cell line derived from normal breast epithelium and immortalized with telomerase (76NTERT cells). Relative levels of transcripts were determined by RT-qPCR in TERT-siSFRP1 cells and the TERT-pSUPER vector control cells. c Similarly, mRNA levels of the genes was compared by RT-qPCR in mouse mammary glands derived from Sfrp1−/− and control Sfrp1+/+ mice. d Breast cancer cells overexpressing SFRP1 (MCF7-SFRP1) and control cells (MCF7-pCDNA) were also compared for relative levels of gene expression by RT-qPCR. The level of SFRP1 mRNA was normalized to the amplification of ACTB mRNA, which was performed in parallel wells for each cell line or tissue. Bars represent mean ± SEM SFRP1/ACTB and are expressed as relative expression of control TERT-pSUPER (b), control mice (c), and MCF7-pCDNA (d). Genes where loss of SFRP1 results in decreased expression are shown in green, those increased are shown in red, and those which are unchanged are shown in white *p < 0.05, **p < 0.01, and ***p < 0.001 (significantly different from control using Student’s t test)

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