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Fig. 1 | Breast Cancer Research

Fig. 1

From: The p52 isoform of SHC1 is a key driver of breast cancer initiation

Fig. 1

Upregulation of SHC1 p46SHC/p52SHC transcript and SHC proteins in human breast cancers. a Quantitative real-time RT-PCR analysis of SHC1 p52SHC/p46SHC transcript levels on a TissueScan cancer survey panel of the breast, colon, prostate, lung, ovary, thyroid, kidney, and liver non-tumor tissue (red bars) and tumor tissue (black bars). Expression is normalized to the median non-cancerous tissue samples. Expression of p52SHC/p46SHC transcript greater than 1.5 is indicated with a number sign, while expression greater than 2 times is indicated with an asterisk. b Western blot analysis of SHC1 isoform expression of paired ER+/PR+/HER2− tumor (T) and non-tumor (N) samples, 5 μg/lane. Lane numbers are indicated at the bottom. c Quantification of data from b panel by normalization to total protein, where *P = 0.008. d Western blot analysis of SHC1 in triple negative ER−/PR−/HER2− paired samples, 10 μg/lane. Lane numbers are indicated at the bottom. e Quantification of data from d panel by normalization to total protein. f INSTA-Blot Breast Tissue OncoPair membrane blotted with both c-terminal total SHC1 and n-terminal p66SHC-specific antibodies. Membranes contain seven paired tumor (T) or non-tumor (N) samples. Locations of three SHC isoforms are indicated. g Western blot analysis of SHC isoform expression (red bands) and β-actin (green) as a loading control in paired normal (N) and DMBA tumor (T) samples from the same rats (n = 3 rats)

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