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Fig. 4 | Breast Cancer Research

Fig. 4

From: Leptin produced by obesity-altered adipose stem cells promotes metastasis but not tumorigenesis of triple-negative breast cancer in orthotopic xenograft and patient-derived xenograft models

Fig. 4

Knockdown of leptin expression in obASCs decreases promotion of metastatic phenotype. a Western blots of signaling pathways activated in BCCS after Transwell co-culture with control shRNA obASCs compared to leptin shRNA obASCs. b RT-qPCR after transwell co-culture with obASC or obASC with leptin shRNA demonstrates decreased mRNA levels of Serpine1, TWIST1, and SNAI2. c Leptin shRNA obASCs decreased migration of BT20, HCC1806, and MCF7 breast cancer cell lines through a 0.4-um transwell membrane. Values reported are the mean of three independent experiments each performed in triplicate. Bars, ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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