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Fig. 3 | Breast Cancer Research

Fig. 3

From: Leptin produced by obesity-altered adipose stem cells promotes metastasis but not tumorigenesis of triple-negative breast cancer in orthotopic xenograft and patient-derived xenograft models

Fig. 3

Adipose stem cells do not affect tumor growth of TNBC xenogafts, but obASCs increase metastases. a Average tumor volume of TNBC cell line BT20 xenograft was consistent for all groups across the time course of the experiment. b Evaluation of metastasis revealed increased area of lung metastases from BT20 tumors grown with obASCs. c Average tumor volume of TNBC PDX TU-BcX-2 K1 was consistent for all groups across the time course of the experiment. d Evaluation of metastasis revealed increased area of metastases in lungs of mice with PDX tumors grown with obASCs in comparison to lnASCs or PDX only. e Flow cytometric analysis of the blood demonstrates increased circulating HLA1+ “human cells” in the blood of the obASC group. There is an increase in cancer stem cell marker CD44+CD24 in the CTCs from the obASC group. There is an increase in circulating CD326+ (epithelial cell adhesion molecule) cells in the obASC group. Caliper measurements were taken every 3 to 4 days until tumor volume reached 750–1000 mm3. Values reported are the mean (n = 5 mice/group). Bars, ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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