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Table 3 NNMT expression reduces the chemo-sensitivities of human breast cancer cells to ADM and PTX

From: Nicotinamide N-methyltransferase enhances chemoresistance in breast cancer through SIRT1 protein stabilization

  IC50 of ADM (μM) IC50 of PTX (nM)
Cell lines Mean ± SEM Fold change Mean ± SEM Fold change
SK-BR-3/Vector 0.12 ± 0.02   12.32 ± 1.20  
SK-BR-3/NNMT-1 0.40 ± 0.05** 3.33 17.41 ± 1.95 1.41
SK-BR-3/NNMT-2 1.00 ± 0.11** 8.33 24.50 ± 2.83* 1.99
MCF7/Vector 0.40 ± 0.05   20.12 ± 1.93  
MCF7/NNMT-1 0.82 ± 0.09* 2.05 28.35 ± 2.14* 1.41
MCF7/NNMT-2 0.92 ± 0.11* 2.30 31.18 ± 3.05* 1.55
MDA-MB-231/NC 1.40 ± 0.09   12.40 ± 1.13  
MDA-MB-231/NNMT shRNA 1# 0.72 ± 0.06** 0.51 7.23 ± 0.78* 0.58
MDA-MB-231/NNMT shRNA 2# 0.61 ± 0.05** 0.44 6.20 ± 0.74* 0.49
  1. Data represent the mean ± SEM of three independent experiments. Statistical significance was detected between the NNMT overexpression or downregulation groups and each matched the control group (SK-BR-3/Vector, MCF/vector or MDA-MB-231/NC)
  2. *p < 0.05, **p < 0.01