Fig. 9

SIRT1 knockdown increases colony formation inhibition by ADM or PTX in NNMT high-expression BCs. The groups that were pretreated with EX527 or SIRT1 siRNA in SK-BR-3/NNMT-2, MCF7, and MDA-MB-231 cells formed fewer colonies (foci > 100 μm) than those without pretreatment after culture for 12 days with ADM or PTX treatment. The group that was pretreated only with DMSO served as the control group for each cell model. a, c, e Representative of three independent experiments. b, d, f The quantification result of a, c, and e, respectively (n = 3) (^**p < 0.01)