Fig. 7
SIRT1 protein and activity levels are decreased by EX527 or SIRT1 siRNA in NNMT high-expression BCs. a SIRT1 mRNA was determined by real-time RT-PCR after SIRT1-specific inhibitor (EX527) (p > 0.05) or SIRT1 siRNA treatment for 48 h (**p < 0.01) (n = 3). b SIRT1 activity levels were determined using a SIRT1 deacetylase fluorometric reagent kit after EX527 or SIRT1 siRNA treatment for 48 h (n = 3) (**p < 0.01). c SIRT1 and acetyl-p53 protein were determined by Western blotting in SK-BR-3/NNMT-2, MCF7/NNMT-2, and MDA-MB-231 after EX527 or SIRT1 siRNA treatment for 48 h. The protein levels were normalized to β-actin. The result is representative of three independent experiments. The group that was pretreated only with DMSO and treated with ddH2O served as the control group for each cell model treated with ADM. The group that was pretreated only with DMSO and treated with DMSO served as the control group for each cell model treated with PTX