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Fig. 6 | Breast Cancer Research

Fig. 6

From: Nicotinamide N-methyltransferase enhances chemoresistance in breast cancer through SIRT1 protein stabilization

Fig. 6

MNA increases SIRT1 protein and activity levels in BCs. a The SIRT1 and acetyl-p53 protein were determined by Western blotting after MNA treatment (0.5 and 1 mM) for 48 h. Protein levels were normalized to those of β-actin. This result is representative of three independent experiments. bd SIRT1 activity levels were determined using a SIRT1 deacetylase fluorometric reagent kit after MNA treatment (0.5 and 1 mM) for 48 h (n = 3) (**p < 0.01). eg SIRT1 mRNA was determined by real-time RT-PCR after MNA treatment (0.5 and 1 mM) for 48 h (n = 3) (p > 0.05). The group without MNA served as the control group for each cell model

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