Fig. 6From: Nicotinamide N-methyltransferase enhances chemoresistance in breast cancer through SIRT1 protein stabilizationMNA increases SIRT1 protein and activity levels in BCs. a The SIRT1 and acetyl-p53 protein were determined by Western blotting after MNA treatment (0.5 and 1 mM) for 48 h. Protein levels were normalized to those of β-actin. This result is representative of three independent experiments. b–d SIRT1 activity levels were determined using a SIRT1 deacetylase fluorometric reagent kit after MNA treatment (0.5 and 1 mM) for 48 h (n = 3) (**p < 0.01). e–g SIRT1 mRNA was determined by real-time RT-PCR after MNA treatment (0.5 and 1 mM) for 48 h (n = 3) (p > 0.05). The group without MNA served as the control group for each cell modelBack to article page