Fig. 5

NNMT increases SIRT1 protein and activity levels in BCs. a–c Overexpression or low expression of NNMT did not significantly change the SIRT1 mRNA level (n = 3) (p > 0.05). d SIRT1, acetyl-p53, and NNMT protein expression was determined by Western blotting. The protein levels were normalized to β-actin. This result is representative of three independent experiments. e–g The SIRT1 activity levels were determined using a SIRT1 deacetylase fluorometric reagent kit (n = 3) (^**p < 0.01). The group with vector or NC served as the control group for each cell model