Fig. 10

SIRT1 knockdown increases apoptosis induced by ADM or PTX in NNMT high-expression BCs. The cells that were pretreated with or without EX527 or SIRT1 siRNA for 24 h were exposed to various concentrations of ADM or PTX for 48 h, and apoptosis was assayed by flow cytometry. a–c The scattergrams of the flow cytometry results from three independent experiments. d, e The quantification results (n = 3) (^**p < 0.01). The group that was pretreated only with DMSO and treated with ddH₂O served as the control group for each cell model treated with ADM. The group that was pretreated only with DMSO and treated with DMSO served as the control group for each cell model treated with PTX