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Fig. 3 | Breast Cancer Research

Fig. 3

From: Identification of three subtypes of triple-negative breast cancer with potential therapeutic implications

Fig. 3

Immunohistochemistry and histological evaluations of neurogenesis and immune markers. a Detection of nerve fibers in C2 TNBC tumors. Nerve fibers in the tumoral stroma, with their typical spindled and wavy morphology, detected with IHC against the axonal marker UCHL1 (60×) and the schwannian marker S100 (60×). UCHL1 and S100 displayed a C2 > C3 profile. b Immune features of C3 TNBC tumors. Tertiary lymphoid structure (TLS) in the vicinity of invasive front of carcinoma, defined by the presence of a germinal center (hematoxylin and eosin staining) (10×), highlighted by follicular dendritic cells marker CD21 (20×). HEV, specialized blood vessels in lymphocytes recruitment, stained by MECA79 (20×), preferentially found in lymphocytes-rich regions of tumors. Plasma cell and B lymphocyte infiltrates were analyzed, respectively, with CD138 and CD20 stainings in the tumoral stroma (40×). Infiltrates were assessed according to recommendations of an international working group, by determining the area occupied by plasma cells or B lymphocytes over the total intratumoral stromal area. TLS, CD21, MECA79, CD138 and CD20 displayed a C3 > C2 profile. Arrows indicate cells expressing each marker (brown). Statistical plots on the right of each picture display the numbers (Pos: positive; Neg: negative) or percentages of marked cells in C2 compared to C3

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