Fig. 4

Rack1 mediates the binding between Src to Anxa2. a Knockdown of Rack1 expression attenuated the interaction between Src and Anxa2 in two drug-resistant cells. Control or Rack1 knockdown cells were lysed, immunoprecipitated with ant-Src or anti-Anxa2 antibodies, and analyzed by Western blotting with anti-Anxa2, Rack1, or Src antibodies. b Silence of Src expression has no significant effect on the binding of Rack1 to Anxa2. Control and Src-silenced cells were lysed, immunoprecipitated with anti-Rack1 or anti-Anxa2 antibodies, and then analyzed by Western blotting using anti-Anxa2, Rack1, or Src antibodies. c Anxa2 knockdown has no significant effect on the interaction between Src and Rack1. Control and Anxa2 knockdown cells were lysed and immunoprecipitated with anti-Src or Rack1 antibodies, followed by Western blotting analysis using anti-Anxa2, Rack1, or Src antibodies