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Fig. 3 | Breast Cancer Research

Fig. 3

From: Rack1 mediates tyrosine phosphorylation of Anxa2 by Src and promotes invasion and metastasis in drug-resistant breast cancer cells

Fig. 3

Anxa2 tyrosine phosphorylation is required for the invasiveness of drug-resistant breast cancer cells. a The expression of Anxa2 and its mutants, Anxa2Y23A and Anxa2Y23D, were effectively rescued in Anxa2-silenced MCF-7/ADR cells as verified by Western blot analysis using anti-Anxa2- or anti-GFP-specific antibodies. β-actin was used as a loading control. b The re-expression of Anxa2WT and the phospho-mimicking Anxa2Y23D mutant, not the phospho-deficient Anxa2 Y23A mutant, in Anxa2-silenced cells rescued the migration and invasion ability. The cell migration and invasion assays were performed by transwell assay. Data are shown as mean ± SD; n = 6; ****P < 0.0001 and nsP > 0.05 indicate no statistical significance. The statistical results are summarized in the following panel

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