Fig. 2

Inhibition of Src kinase blocked Anxa2 phosphorylation and decreased invasiveness of drug-resistant breast cancer cells. a Inhibition of Src kinase by using Src kinase inhibitors blocked the phosphorylation of Anxa2 at Tyr23 site in drug-resistant cells. β-actin was used as the loading control. b The cell invasion ability was significantly suppressed in Src inhibitor-treated group compared with the control group. Data are shown as mean ± SD; n = 6; ****P < 0.0001 compared with DMSO control. c Src knockdown evidently inhibited EGF-induced tyrosine phosphorylation of Anxa2 in drug-resistant cells. β-actin was used as the loading control. d Knockdown of Src expression inhibited cell migration and invasion ability. The assays were repeated three times. Data are shown as mean ± SD; n = 6; **P < 0.01, ***P < 0.001, and ****P < 0.0001 versus control