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Fig. 1 | Breast Cancer Research

Fig. 1

From: Rack1 mediates tyrosine phosphorylation of Anxa2 by Src and promotes invasion and metastasis in drug-resistant breast cancer cells

Fig. 1

Rack1 is required for Anxa2 Tyr23 phosphorylation and enhanced invasiveness of drug-resistant breast cancer cells. a Rack1 knockdown decreased the basal levels of phosphorylated Anxa2 in two drug-resistant cells. Western blotting analysis of the total and phosphorylated Anxa2 expression in MCF-7/ADR and MDA-MB-468/ERP cancer cells transfected with negative control or siRNAs targeting Rack1 for 72 h; β-actin was used as the loading control. b Rack1 knockdown inhibited EGF-induced Tyr23 phosphorylation of Anxa2. c Knockdown of Rack1 expression in two drug-resistant cells significantly decreased cell migration ability as measured by wound healing assay. Data are shown as mean ± SD; n = 6; ****P < 0.0001 versus control. Statistical analysis was performed by two-way ANOVA. d Knockdown of Rack1 expression attenuated the migration and invasion ability in two drug-resistant cells. For cell migration assay, 1 × 105 cells in 200 μL of serum-free medium were loaded into the upper chamber. For cell invasion assay, 2.5 × 105 cells in 200 μL serum-free medium were loaded into the upper chamber coated with Matrigel. The statistical results are summarized in the right panel. Data as mean ± SD; n = 6; ****P < 0.0001 compared with the control group

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