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Fig. 4 | Breast Cancer Research

Fig. 4

From: Glutamine to proline conversion is associated with response to glutaminase inhibition in breast cancer

Fig. 4

Utilization of [5-13C] Gln in untreated luminal-like (MAS98.06) and basal-like (MAS98.12) tumor xenografts. a 13C labeling patterns in MAS98.06 and MAS98.12 tumors after administration of [5-13C] Gln. MAS98.06 tumors (blue) use [5-13C] Gln to produce [5-13C] Glu, [5-13C] Pro, [1-13C] Ala, [1-13C] Glu, and [1-13C] Lac (gray, only borderline significant). They also store a significant amount of [5-13C] Gln in the tumors. MAS98.12 tumors (pink) take up [5-13C] Gln and use it for production of [5-13C] Glu, [1-13C] Lac and [5-13C] Ala (gray, only borderline significant). b Amount of 13C-labeled metabolites in the tumors, calculated by subtracting natural abundance spectra from 13C-enriched spectra. Stars (*) indicate that there is a significantly higher amount of the metabolite in 13C-enriched samples compared to natural abundance samples, while up arrowheads (^) indicate borderline significance. The total amount of 13C-labeled metabolites were not significantly different between the two models. ^p < 0.1, *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations: Ala, alanine; Gln, glutamine; GLS1, glutaminase; Glu, glutamate; Lac, lactate; Pro, proline; Pyr, pyruvate; TCA, tricarboxylic acid

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