Skip to main content
Fig. 4 | Breast Cancer Research

Fig. 4

From: The opposing effects of interferon-beta and oncostatin-M as regulators of cancer stem cell plasticity in triple-negative breast cancer

Fig. 4

Restoration of IFN-β signaling represses OSM-mediated CSC properties and SNAIL expression. a Lentiviral transduction of IFN-β in BT549-OSM cells restores canonical IFN-β signaling comparably to BT549-GFP cells and represses OSM-mediated SNAIL expression independently of STAT3 activation, as demonstrated by Western analysis. The lines indicate separate Western blots were run using matched samples. b IFN-β overexpression in BT549-OSM cells restores ISG mRNA expression comparably to BT549-GFP cells, as demonstrated by qRT-PCR (STAT1, STAT2, IRF9, SOCS1, MX1, OAS1) but does not restore IRF1 and IFI16 expression (***P < 0.001, *****P < 0.00001) ± SEM, n = 3. c IFN-β overexpression in BT549-OSM cells significantly represses tumor sphere formation comparably to BT549-GFP (stem cell frequencies: 1:Inf (Infinity) BT549-GFP, 1:11 BT549-OSM, 1:Inf BT549-OSM-IFN-β) (***P < 0.001) ± SD, n = 5. d IFN-β overexpression significantly represses OSM-mediated tumor initiation in vivo when engrafted subcutaneously at 20,000 or 200,000 cells/injection (nu/nu female mice) as demonstrated by decreased tumor weights at day 21 (end of experiment) (one-way ANOVA, ****P < 0.0001) ± SD, n = 5 mice

Back to article page