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Fig. 3 | Breast Cancer Research

Fig. 3

From: BRCA1 mutations attenuate super-enhancer function and chromatin looping in haploinsufficient human breast epithelial cells

Fig. 3

BRCA1185delAG/+ MCF10A cells recapitulate H3K27ac changes in BRCA1mut/+ HMECs. a Relative H3K27ac levels at six BRCA1mut/+-attenuated super-enhancers. The loci are named after the most proximal genes. The graph is an average of five independent experiments with two WT MCF10A clones (colored blue) and two BRCA1185delAG/+ MCF10A clones (colored red). b Western blot of H3K27ac in WT and BRCA1185delAG/+ MCF10A clones. Total histone H3 was used as the loading control. c Relative H3K27ac levels at BRCA1mut/+-attenuated super-enhancers. The graph is an average of three independent experiments with two WT MCF10A clones, two BRCA1C61G/+ clones, two BRCA1R71G/+ clones, and two BRCA1185delAG/+ clones. *P < 0.05 by two-tailed t test. Error bars represent s.e.m

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