Fig. 6
From: The pro- and anti-tumor roles of mesenchymal stem cells toward BRCA1-IRIS-overexpressing TNBC cells
The opposing roles of MSCs in IRISOE TNBC tumors. a Schematic representation of the assays presented in (b, c). b Tumor volumes during the 10 weeks following injection in the 2nd thoracic mammary gland of athymic mice of 2 × 106 IRIS291 (yellow), 2 × 106 IRIS291 + 2 × 105 human MSCs (red), 2 × 106 IRIS293 (light blue), or 2 × 106 IRIS293 + 2 × 105 human MSCs (dark blue). c Comparison of percentage of survival in athymic mice injected with IRIS291 (upper, black) and IRIS293 alone (lower, black) vs. mice injected with IRIS291 admixed with MSCs (upper, red) or IRIS293 admixed with MSCs (lower, green). d Percentage of GFP+ cells at days 0–8 in cultures of MDA-468/shCtrl cells + MSCs relative to MDA-468/shCtrl cells (black), and MDA-468/shIRIS cells + MSCs relative to MDA-468/shIRIS cells (red). Representative fluorescence and bright field images of MDA-468/shCtrl (e1 and 2), MDA-468/shCtrl + MSCs (e3 and 4), MDA-468/shIRIS (e5 and 6), or MDA-468/shIRIS + MSCs (e7 and 8). Assay was done three different times in four replicates. Scale bar = 400 μm. f Schematic representation of the assays presented in (g, h). g Tumor volumes during the 60 days following injection of athymic mice 2nd thoracic mammary gland with 1 × 106 MDA-231/shCtrl cells (yellow), 1 × 106 MDA-231/shCtrl cells + 1 × 105 human MSCs (red), 1 × 106 MDA-231/shIRIS cells (light blue), and 1 × 106 MDA-231/shIRIS cells + 1 × 105 human MSCs (dark blue). h Upper: comparison of the percentage of survival of mice in g injected with MDA-231/shCrtl (green) and MDA-231/shIRIS (black). Lower: comparison of percentage survival of mice in g injected with MDA-231/shCtrl admixed with MSCs (dashed) or without MSCs (solid). i Schematic representation of the assays presented in (j, k). j Tumor volumes during 40 days following injection of BALB/c mice 2nd thoracic mammary gland with 5 × 105 4T1/shCtrl cells (yellow), 5 × 105 4T1/shCtrl cells + 5 × 104 mouse MSCs (red), 5 × 105 4T1/shIris1 cells (light blue), and 5 × 105 4T1/shIRIS cells + 1 × 104 mouse MSCs (dark blue). k Upper: comparison of the percentage of survival of mice in g injected with 4T1/shCrtl cells (black), 4T1/shIris1 cells (red) or 4T1/shIris2 cells (green). Lower: comparison of the percentage of survival of mice in g injected with 4T1/shCtrl cells admixed with MSCs (red) or without MSCs (blue)