Fig. 6

MIR2052HG regulates LMTK3 transcription by regulating EGR1 binding to its motif in LMTK3 gene. a, b EGR1 regulates LMTK3 expression in MCF7/AC1 (a) and CAMA-1 (b) cells. c The EGR1 binding sites at the genomic location of the LMTK3 gene locus are indicated in the diagram. d, e ChIP analysis demonstrates binding of EGR1 to LMTK3 gene locus in MCF7/AC1 (d) and CAMA-1 (e) cells. IgG serves as a control. Error bars represent SEM of two independent experiments in triplicate; **p < 0.01. f Dual RNA-DNA-FISH demonstrates that MIR2052HG transcripts (green signal) fail to localize to the LMTK3 gene locus (red signal) in EGR1 knockdown MCF7/AC1 and CAMA-1 cells