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Fig. 3 | Breast Cancer Research

Fig. 3

From: The lncRNA MIR2052HG regulates ERα levels and aromatase inhibitor resistance through LMTK3 by recruiting EGR1

Fig. 3

LMTK3-mediated MIR2052HG effect on regulation of ESR1 transcription and ERα protein stability. ab Overexpression of LMTK3 in MIR2052HG knocked-down MCF7/AC1 (a) and CAMA-1 (b) cells reversed ERα protein and mRNA levels and, in turn, decreased AKT phosphorylation and FOXO3 level. Knockdown efficiency was determined by qRT-PCR. Overexpression of LMTK3 was determined by western blot analysis. Protein levels were quantified using ImageJ (right panel). Protein levels were normalized to GAPDH, and the normalized protein level in Neg+EV was set to 1. Error bars represent SEM. The significant difference between Neg+EV and all the other samples is indicated by **p < 0.01. c Overexpression of LMTK3 in MIR2052HG knocked-down cells reduced the ubiquitination of ERα. 293 T cells were transfected with HA-Ub plasmid and FLAG-ERα plasmid and then transfected with either the MIR2052HG ASOs or LMTK3 plasmid followed by MG132. ERα proteins were immunoprecipitated and analyzed by western blot analysis (left panel). Overexpression of LMTK3 was determined by western blot (middle panel). Knockdown efficiency was determined by qRT-PCR (right panel)

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