Fig. 4

Mesenchymal-like cells are more sensitive to GSK3β inhibitors as compared to the epithelial cells. a MCF10A, a normal breast cell line, and MCF7 epithelial cells were more resistant to GSK3β inhibitors as compared to SUM159, a mesenchymal cell line. b A co-culture experiment was performed by mixing an equal proportion of green HMLER epithelial cells and red HMLER-Snail mesenchymal-like cells and treating them with GSK3β inhibitors. Following treatment with GSK3β inhibitors, the percentage of epithelial cells increased as compared to the percentage of mesenchymal cells. c HMLE and HMLE-Snail cells were treated with a dose range of the tested inhibitors, and viability was assessed by MTT assay and the heatmap summarizes the differential selectivity screen