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Fig. 8 | Breast Cancer Research

Fig. 8

From: Osteoblasts are “educated” by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment

Fig. 8

EO cells produce altered amounts of cytokines compared with normal osteoblasts. Differences in the levels of soluble factors between EOs and “uneducated” OBs were analyzed using a Raybiotech Quantibody Array Q4000, which surveys the protein expression of 400 cytokines. Conditioned medium was prepared from vehicle-treated MC3T3-E1, EO-HMEC, EO-231, EO-BRMS, or EO-MCF7 cells grown to confluence and analyzed for cytokine content via a RayBiotech Mouse Quantibody Array. Three individual conditioned media batches per analyte, per condition, were analyzed. A literature search of the biological properties of the top 10 proteins with altered levels in EOs compared to “uneducated” OBs was then carried out. Among the top 10 proteins with upregulated cytokine levels in EO cells, we focused on NOV. Among the top 10 proteins with downregulated levels in EO cells, we focused on decorin. a Decorin and b NOV cytokine expression. *P < 0.05; #P < 0.05; ****P < 0.0001, ####P < 0.0001

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