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Fig. 4 | Breast Cancer Research

Fig. 4

From: MEDI3039, a novel highly potent tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) receptor 2 agonist, causes regression of orthotopic tumors and inhibits outgrowth of metastatic triple-negative breast cancer

Fig. 4

MEDI3039 inhibited tumor growth and extended animal survival in the MB231T mammary fad pad model. a Design of the experiment. MEDI3039 was administered weekly, for 5 weeks at indicated doses. Five mice in the control and 0.3 mg/kg group were sacrificed for histology analysis and 10 mice per group were followed for tumor growth and survival. b Immunohistochemistry analysis of tumor samples from MEDI3039 (0.3 mg/kg) or vehicle-injected mice. Samples were stained with either Ki67 and DAPI or CC3 (cleaved caspase 3) and DAPI. Bar = 100 μm. c Quantitative analysis of signal intensity of Ki67 and CC3, both normalized with DAPI. Data is shown as median with IQR. Numbers of mice examined was 5 (vehicle control.) and 4 (MEDI3039 0.3 mg/kg). p value was obtained by Mann–Whitney test. d Tumor growth curve in each treatment group. The bottom panel shows averages for all mice at each dose. One-way ANOVA was used to compare statistical significance between different groups. e Tumor growth inhibition curve. Data is shown as median % tumor inhibition of 0.03, 0.1, and 0.3 mg/kg MEDI3039 groups, compared with the vehicle control group. f Survival curve of mice treated with MEDI3039 at indicated doses. Median survival was 38 days (vehicle control group), 44 days (MEDI3039, 0.03 mg/kg group), 74 days (MEDI3039, 0.1 mg/kg group), 79.5 days (MEDI3039, 0.3 mg/kg group). p value, HR (hazard ratio), and 95% of CI (confidence interval) were obtained by log-rank (Mantel–Cox) test, compared with the vehicle control group

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