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Fig. 5 | Breast Cancer Research

Fig. 5

From: MiR-1287-5p inhibits triple negative breast cancer growth by interaction with phosphoinositide 3-kinase CB, thereby sensitizing cells for PI3Kinase inhibitors

Fig. 5

a–c Clonogenic assay of the cell lines SUM159, MDA-MB-231, and BT549 after transient silencing of the putative miR-1287-5p target PIK3CB leads to a similar phenotype compared to miR-1287 overexpression in the cell lines. Cells develop less colonies after PIK3CB silencing (a, b) and PIK3CB silencing also leads to a G1 Phase Arrest (c) in all four cell lines. d–g SUM159 and BT549 cells treated with two different concentrations of PI3Kinase inhibitors in combination with control scrambled RNA (10 μM of Allstar negative control) or the miR-1287-5p mimics (10 μM of miR-1287-5p mimics) (d, e) CAL101 (Idelalisib) and f, g BYL719 (Alpelisib). Cells treated with miR-1287-5p mimic are more sensitive to CAL-101 and BYL719 treatment in both tested cell lines compared to cells treated with the scrambled control RNA. *p < 0.05

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Breast Cancer Research

ISSN: 1465-542X

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