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Fig. 4 | Breast Cancer Research

Fig. 4

From: MiR-1287-5p inhibits triple negative breast cancer growth by interaction with phosphoinositide 3-kinase CB, thereby sensitizing cells for PI3Kinase inhibitors

Fig. 4

Target identification of miR-1287-5p in triple negative breast cancer cells. a qRT-PCR confirmed a significant downregulation of the PIK3CB mRNA in all four tested triple negative BC cell lines after forced ectopic miR-1287-5p overexpression after 48 h of transfection. b Western blot analysis confirmed a significant downregulation of PIK3CB on protein level after transient transfection of miR-1287-5p in all tested cell lines (SUM159, BT549, MDA-MB-231, and MDA-MB-468) after 48 h of transfection. Relative quantification (numbers above the lanes) of protein lanes was performed using ImageJ. c Predicted miR-1287-5p interaction site within 3′ untranslated region of PIK3CB mRNA. Two PIK3CB constructs were generated as indicated (WT = miR-1287 wild-type interacting site and MT = mutated interacting site). d Luciferase activity after co-transfection of the PIK3CB wild-type or mutated constructs and control/miR-1287-5p mimetic in HEK cells. Three independent biological experiments were performed, and the means and standard deviations are shown. e High-PIK3CB expression is associated with poor clinical outcome in 1005 BC patients of a TCGA dataset.*p < 0.05

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