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Fig. 3 | Breast Cancer Research

Fig. 3

From: p53 controls the plasticity of mammary luminal progenitor cells downstream of Met signaling

Fig. 3

p53-deficient luminal progenitors are stimulated by HGF but fail to acquire basal-specific markers. a Microphotographs of secondary mammospheres derived from control and mutant Lu-pos cells cultured in the absence of presence of HGF for 10 days. Bar, 200 μm. b Percentages of sphere-forming cells after consecutive passages of 5000 control and mutant Lu-pos cells grown in the absence or presence of HGF. MS1 and MS2 refer to primary and secondary mammospheres. Data are the mean ± SEM of 4 distinct assays. *p ≤ 0.05, **p ≤ 0.01. c qPCR analysis of Krt5 expression in MS1 and MS2 spheres derived from control and mutant Lu-pos cells cultured in the absence or presence of HGF. Data are the mean ± SEM of 4 separate preparations. **p ≤ 0.01. d Double K5/K8 staining of sections through secondary spheres derived from control and mutant Lu-pos cells cultured in the absence or presence of HGF. Bar, 40 μm. e Comparative expression levels of lineage-specific genes in MS2 spheres derived from control and mutant Lu-pos cells untreated or treated with HGF. qPCR data are expressed as log2 ratios of values normalized to Gapdh. Comparator values were those obtained with untreated cells. Data are the mean ± SEM of 3 distinct preparations

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