Fig. 2

rhEpo enhances radiation-induced phenotype conversion in vitro. a MCF7-ZsGreen-cODC, b T47D-ZsGreen-cODC, c MDA-MB-231-ZsGreen-cODC, and d SUM150PT-ZsGreen-cODC were depleted of intrinsic BCICs by sorting for ZsGreen-negative cells. The cells were plated and the following day were treated with vehicle (light gray), 1 IU/ml rhEpo (dark gray), or 10 IU/ml rhEpo (black). One hour later, the cells were subjected to 0, 4, and 8 Gy or five daily doses of 3 Gy. Six days after rhEpo treatment, the cells were harvested and analyzed by flow cytometry. Graph represents the average %ZsGreen-positive cells from at least four independent repeats ± SEM. Statistical significance was determined by Student’s t tests. * p < 0.05, ** p < 0.01