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Fig. 1 | Breast Cancer Research

Fig. 1

From: Serum erythropoietin levels, breast cancer and breast cancer-initiating cells

Fig. 1

rhEpo enriches for the intrinsic BCICs in vitro. a MCF7-ZsGreen-cODC, b T47D-ZsGreen-cODC, and c MDA-MB-231-ZsGreen-cODC were plated into sphere cultures. d SUM150PT-ZsGreen-cODC were plated in adherent conditions. a–d The cells were treated with 1 IU/ml rhEpo (gray bars) or vehicle (black bars) and 1 h later were subjected to 0, 4, and 8 Gy or five daily doses of 3 Gy. Six days after Epo treatment, the cells were harvested and analyzed by flow cytometry. The graph represents the average %ZsGreen-positive BCICs from at least three independent repeats ± SEM. * p < 0.05, ** p < 0.01

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