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Fig. 2 | Breast Cancer Research

Fig. 2

From: Using an in-vivo syngeneic spontaneous metastasis model identifies ID2 as a promoter of breast cancer colonisation in the brain

Fig. 2

Downregulation of Id2 or Aldh3a1 expression impairs tumour growth in the brain. a RT-qPCR analysis of Id2 (left panel) and Aldh3a1 (right panel) expression in 4T1-Luc cells transduced with lentiviral non-targeting control (shNTC) small hairpin RNA (shRNA) or shRNAs targeting Id2 (shId2) or Aldh3a1 (shAldh3a1), n = 3. b 1 × 104 4T1-Luc cells were inoculated intracranially into BALB/c mice, n = 4–6 mice per group ± SEM. **p < 0.01, *p < 0.05, Kruskal-Wallis test. At day 9 whole brains were collected at necropsy and subjected to ex-vivo IVIS imaging (left panel), and IVIS images are shown (right panel). c Brains from b were fixed, paraffin embedded, and sectioned for histological (H&E) quantification of tumour burden (left panel). Mean ± SEM. *p < 0.05, Kruskal-Wallis test. Right panel shows representative histological sections. Scale bar = 2.5 mm

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