Fig. 3
Long-term oestrogen deprivation therapy is associated with cell cycle, senescence, epigenetic regulation, and extracellular matrix (ECM)-associated pathways. Differentially expressed genes between pre-treatment and long-term treated dormant (a) and resistant (b) tumours were determined. Heat-maps showing change in downregulated and upregulated gene expression in dormant (a) and resistant (b) samples. Each column represents a sample and each row a gene. Colours are log2 mean-centred values with red indicating high values and blue indicating low expression. Bar plots on top of heat-maps represent the time on treatment (days) for each sample. c,d Graphs show dynamic changes in mean expression of differentially expressed genes in response classes. ***p < 0.001; **p < 0.01; *p < 0.05